学术交流
生物节律学术报告
1:题目：Circadian protein kinase activity: both a target and a mediator of circadian regulation
时间：2013年7月4日14:00
地点：中山大学东校区生命科学学院大楼227课室
报告人： Dr. Jeffrey L. Price, Assoc. Prof., School of Biological Sciences, University of Missouri at Kansas City
主持人：郭金虎教授, 中山大学生命科学学院生物昼夜节律实验室
Dr. Price’s lab has been using proteomic and genetic approaches to identify proteins and cellular pathways that interact with DBT, the principal kinase targeting circadian transcription factor PER for phosphorylation and degradation. The proteomic approach identified a previously unstudied protein that interacts with DBT. RNAi-mediated knock-down of its mRNA produced behavioral arrhythmicity, high levels of hypophosphorylated nuclear PER and altered DBT. In photoreceptors, the protein accumulates rhythmically in PER- and DBT-dependent cytosolic foci. Finally, structural analyses demonstrated that the protein is a noncanonical FK506-binding protein with an inactive peptide prolyl-isomerase domain and tetratricopeptide repeats. We have named this novel circadian protein Bride of DBT (bdbt) and established it as a key mediator of DBT’ s effects on PER, most likely in cytosolic foci that regulate PER nuclear accumulation. Analysis of circadian period-altering mutants of DBT has suggested that at least one of them alters period by affecting a protein interaction domain, while analysis of a catalytically inactive DBT (DBTK/R) has revealed a circadian role for DBT in suppressing cell death pathways. Caspases are activated in a circadian manner in flies expressing DBTK/R. This last result has implications for circadian clocks in regulation of lifespan and healthspan.
2:题目：Regulation of locomotor rhythm and sleep in Drosophila melanogaster
时间：2013年7月4日15:00
地点：中山大学东校区生命科学学院大楼227课室
报告人： 赵章武 教授, 中国农业大学 昆虫学系
主持人：郭金虎 教授, 中山大学生命科学学院生物昼夜节律实验室
赵章武教授实验室用黑腹果蝇作物模式动物，主要从事生物活动节律和睡眠的行为和调控机理研究。主要包括以下几个方面：1. 核心钟神经元调控生物活动节律和睡眠，但涉及到许多中间因子参与调控。我们的目标之一是探索这些因子，以及它们之间的相互作用机理；2. 目前发现一些MiRNA参与调控上述行为，我们的长期目标是挖掘参与生物钟调控的MiRNA以及作用机制；3. 核心钟蛋白结构的研究；4. 探索可能参与生物活动节律的细胞信号通路。
时间：2013年7月4日14:00
地点：中山大学东校区生命科学学院大楼227课室
报告人： Dr. Jeffrey L. Price, Assoc. Prof., School of Biological Sciences, University of Missouri at Kansas City
主持人：郭金虎教授, 中山大学生命科学学院生物昼夜节律实验室
Dr. Price’s lab has been using proteomic and genetic approaches to identify proteins and cellular pathways that interact with DBT, the principal kinase targeting circadian transcription factor PER for phosphorylation and degradation. The proteomic approach identified a previously unstudied protein that interacts with DBT. RNAi-mediated knock-down of its mRNA produced behavioral arrhythmicity, high levels of hypophosphorylated nuclear PER and altered DBT. In photoreceptors, the protein accumulates rhythmically in PER- and DBT-dependent cytosolic foci. Finally, structural analyses demonstrated that the protein is a noncanonical FK506-binding protein with an inactive peptide prolyl-isomerase domain and tetratricopeptide repeats. We have named this novel circadian protein Bride of DBT (bdbt) and established it as a key mediator of DBT’ s effects on PER, most likely in cytosolic foci that regulate PER nuclear accumulation. Analysis of circadian period-altering mutants of DBT has suggested that at least one of them alters period by affecting a protein interaction domain, while analysis of a catalytically inactive DBT (DBTK/R) has revealed a circadian role for DBT in suppressing cell death pathways. Caspases are activated in a circadian manner in flies expressing DBTK/R. This last result has implications for circadian clocks in regulation of lifespan and healthspan.
2:题目：Regulation of locomotor rhythm and sleep in Drosophila melanogaster
时间：2013年7月4日15:00
地点：中山大学东校区生命科学学院大楼227课室
报告人： 赵章武 教授, 中国农业大学 昆虫学系
主持人：郭金虎 教授, 中山大学生命科学学院生物昼夜节律实验室
赵章武教授实验室用黑腹果蝇作物模式动物，主要从事生物活动节律和睡眠的行为和调控机理研究。主要包括以下几个方面：1. 核心钟神经元调控生物活动节律和睡眠，但涉及到许多中间因子参与调控。我们的目标之一是探索这些因子，以及它们之间的相互作用机理；2. 目前发现一些MiRNA参与调控上述行为，我们的长期目标是挖掘参与生物钟调控的MiRNA以及作用机制；3. 核心钟蛋白结构的研究；4. 探索可能参与生物活动节律的细胞信号通路。
